The incidence of NHL has increased strikingly during the last four decades, nearly doubling from 1970 to 1990. Non-Hodgkin's lymphoma (NHL) is a heterogeneous group of lymphoid malignancies characterized by an abnormal clonal proliferation of B cells, T cells or both. Taken together, these results demonstrate that miR-148b increased the radiosensitivity of NHL cells probably by promoting radiation-induced apoptosis, which suggests that miR-148b plays an important role in the response of NHL to ionizing radiation. An apoptosis assay showed that miR-148b enhanced apoptosis of Raji cells after irradiation. MiR-148b did not affect the cell cycle profile of post-radiation Raji cells compared with controls. A clonogenic assay demonstrated that miR-148b sensitized Raji cells to radiotherapy. A proliferation assay showed that miR-148b could inhibit the proliferation of Raji cells before and after radiation. Transient transfection experiments showed that miR-148b was up-regulated by miR-148b mimic and down-regulated by miR-148b inhibitor in the Raji cells. A quantitative real-time polymerase chain reaction (PCR) assay confirmed the up-regulation of miR-148b after radiation. Among the differentially expressed miRNAs, miR-148b was up-regulated 1.53-fold in response to radiation treatment. A total of 20 differentially expressed miRNAs were identified including 10 up-regulated and 10 down-regulated (defined as P < 0.05). Microarray was employed to compare the miRNA expression profiles in B cell lymphoma cell line Raji before and after a 2-Gy dose of radiation. This study aimed to explore the role of miRNAs in non-Hodgkin's lymphoma (NHL) radiosensitivity. Growing evidence has demonstrated that microRNAs (miRNAs) play an important role in regulating cellular radiosensitivity.
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